Simplified rAAV lysate purification for complex analytics of upstream samplesShare this post 0Bioprocess experts from Sartorius BIA Separations and BridgeBio Gene Therapy illustrate an approach with potential to expedite AAV upstream analytics and lower overall process development costs. Optimising upstream production of recombinant adeno-associated viruses (AAV) is essential for reducing costs, improving quality and enhancing efficiency. This process occurs during the complex upstream phase, necessitating precise analytical methods and relatively pure samples to determine capsid ratios. This method is designed to purify low-volume rAAV upstream samples, suitable for various analytics requiring specific purity and concentration levels. rAAV samples are acidified, vortexed, incubated, centrifuged, and loaded onto the plate.