Called OneCell CUT&Tag, the method simultaneously profiles chromatin states, gene activity and cell-surface markers from the same cell, even when only one cell is available. OneCell CUT&Tag adapts the principles of CUT&Tag, or cleavage under targets and tagmentation, for extremely limited input. Subject of Research: Simultaneous single-cell profiling of chromatin states, transcriptomes and surface markers using OneCell CUT&Tag, with applications in mammary-gland cell-fate conversion and zygotic gene regulation. “Simultaneous single-cell profiling of chromatin, transcriptome and surface markers with OneCell CUT&Tag captures epigenomic reprogramming.” https://doi.org/10.1038/s41587-026-03259-1Image Credits: AI GeneratedDOI: https://doi.org/10.1038/s41587-026-03259-1Keywords: OneCell CUT&Tag, single-cell sequencing, epigenomics, chromatin, transcriptome, surface markers, H3K27me3, mammary gland, transdifferentiation, cell fate, zygotic gene expression, developmental biology