A new study published in the Journal of Proteome Research revealed novel protein markers alpha-1-acid glycoprotein 1 (AGP1) and zinc-alpha-2-glycoprotein (ZAG) to show potential as urinary biomarkers for detecting early metabolic changes linked to prediabetes and progression to type 2 diabetes (T2D). Thus, studies have been exploring less invasive tools capable of detecting early metabolic disturbances before full disease onset. Using enzyme-linked immunosorbent assay (ELISA) testing this study included larger cohort of 91 control participants and 68 individuals with prediabetes. These findings indicate that urinary proteins may reflect early metabolic dysfunction associated with insulin resistance and diabetes progression. Discovery of novel protein biomarkers in urine for diagnosis of prediabetes using label-free proteomics.