Hawon Yoon (left; UNC Chapel Hill, Department of Cell Biology and Physiology, NC, USA) is a research technician in Berfin Azizoglu’s lab at UNC Chapel Hill. She has a bachelor’s degree from UNC Chapel Hill, with a double major in Biology and Psychology and a minor in Creative Writing. Aashita Rajput (right; UNC Chapel Hill, Department of Cell Biology and Physiology) is a research assistant and lab manager in Azizoglu’s lab at UNC Chapel Hill. Please provide a short summary of your paperThis paper introduces an accessible immunofluorescence staining protocol for vibratome-generated, multi-cell thick, free-floating tissue sections that incorporates heat-mediated antigen retrieval. As we began our work, we noted a shortage of techniques that enable visualization and quantitative assessment of the three-dimensional liver lobule.