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Nanoscopy reveals heparan sulfate clusters as docking sites for SARS-CoV-2 attachment and entry
['Sue Han', 'Xin Wang', 'Tiansheng Li', 'Ammar Mohseni', 'Ivan Kosik', 'Chung Yu Chan', 'Alberto Domingo López-Muñoz', 'Jessica Matthias', 'Reid Suddaby', 'Zhixiong Wang']
eLife: latest articles
Clusters of ~6–137 HS molecules, not single HS molecules, are the docking site for SARS-CoV-2 attachment (Figures 3 and 5).
We propose that HS outcompetes ACE2, which extends less than 10 nm from the PM (Yan et al., 2020), for virion attachment (Clausen et al., 2020; Walls et al., 2020; Wrapp et al., 2020; Liu et al., 2021).
Our model (Figure 5I) differs in three aspects from the generally accepted current model (Jackson et al., 2022; Clausen et al., 2020; Zhang et al., 2020).
Although light microscopic imaging showed S and hACE2 at the PM (Wang et al., 2020; Bayati et al., 2021), limited resolution precluded demonstrating a direct association.
While some studies using genome-wide CRISPR screening to identify genes involved in SARS-CoV-2 reveal genes for HS biosynthesis, others do not (Chan et al., 2023; Wang et al., 2021; Baggen et al., 2021; Rebendenne et al., 2022; Wei et al., 2021; Zhu et al., 2021).