The core premise is developing a biosensor that can detect MPs efficiently and then integrating it with Raman spectroscopy to enhance identification capabilities. Traditional MP detection techniques predominantly rely on physical and chemical analyses such as microscopic enumeration, Fourier-Transform Infrared Spectroscopy (FTIR), and Raman spectroscopy. The integration with Raman spectroscopy allowed quantification and polymer-type identification, which is crucial for understanding MP sources and environmental impacts. ConclusionThis study underscores the transformative potential of microbial biosensors in environmental pollution detection, specifically for microplastics. Detection of microplastics pollution using a green fluorescent protein-based microbial biosensor coupled with Raman spectroscopy.