Figure 2 with 1 supplement with 1 supplement see all Download asset Open asset Combining AMPK activity and fatty acid synthesis to avoid senescence. (F) Size of colonies formed in 24 hr on a YPD plate by log phase and age-matched wild type and A2A. While young A2A cells formed slightly smaller colonies than did wild-type cells, the colonies formed by aged A2A cells were much larger than those formed by wild-type cells of equivalent replicative age (Figure 2F). Indeed, aged A2A cells formed colonies of similar size to young A2A cells, showing equivalent fitness, whereas most aged wild-type cells divided only once or not at all across the 24-hr assay period. This demonstrates that A2A cells maintain excellent fitness late in life, whereas the fitness of aged wild-type cells is very low.