This same-day imaging approach has the potential to save valuable time in guiding treatment decisions and reduce unnecessary exposure to ineffective therapies. Triple-negative breast cancer (TNBC) is an aggressive subtype that accounts for approximately 24 percent of newly diagnosed breast cancer cases. Similarly, urothelial bladder carcinoma (UBC) represents the most prevalent malignancy of the urinary tract, with urothelial bladder cancer comprising about 90 percent of cases. Nectin-4 expression in human TNBC and UBC cell lines was assessed by flow cytometry and immunofluorescence. Next, immuno-PET imaging and biodistribution studies were conducted in mice bearing subcutaneous xenografts with varying levels of nectin-4 expression.