StemFit Basic 03 is an animal-origin free, defined medium for human pluripotent stem cell culture. Capable of maintaining human embryonic stem cell and iPS cell under feeder-free culture conditions, StemFit 03 is widely cited for both clinical research and manufacturing to GMP-regulated standards. iMatrix-511 is a cell culture matrix compatible with a wide variety of cell types and is well suited for pluripotent stem cells. Ideal for feeder-free cell culture, iMatrix-511 was used in this study to maintain pluripotent colony morphology and adhesion, which is important for consistent cell expansion and differentiation outcomes. This expertise in cell culture and the ECM allows Amsbio to partner with clients in tailoring cell systems to enhance organoid and spheroid screening outcomes using a variety of 3D culture systems, including organ-on-a-chip microfluidics.