Not only does temperature regulation impact bees’ foraging abilities under a range of weather conditions, but a bee’s body temperature can directly impact flight capability. Prior research shows the importance of thermogenesis in honey bees, highlighting the role of octopamine signaling and octopamine receptors in this process. While neonics have been previously recognized as neurotoxic agents, their implications on body temperature in honey bees has not been fully evaluated. Additional analyses include the determination of octopamine content in honey bees and the effect of dinotefuran enantiomers on genes and the homing ability of honey bees. The results reveal that Rac-dinotefuran-treated honey bees have the highest body temperatures when compared to untreated bees, while all bees treated with thiamethoxam and dinotefuran exhibit elevated body temperatures.